Chlorophyll Expression Varies with Development State in the Temperature-Sensitive ch4 Mutation of Melilotus alba

Chlorophyll Expression Varies with Development State in the Temperature-Sensitive ch4 Mutation of Melilotus alba.

The ch4 mutant of sweetclover (Melilotus alba) has previously been demonstrated to be temperature- and photoperiod-sensitive for the accumulation of chlorophyll. Pigment content of ch4 mutant leaves was examined as a function of trifoliolate mass, an index of leaf development. Inhibition of chlorophyll accumulation caused by increased growth temperature or decreased photoperiod can be attenuate...

A Temperature-Sensitive Chlorophyll β-Deficient Mutant of Sweetclover (Melilotus alba)

A Temperature-Sensitive Chlorophyll b-Deficient Mutant of Sweetclover (Melilotus alba).

The ch4 mutant of sweetclover (Melilotus alba) has previously been demonstrated to be partially deficient in chlorophyll and to have a higher ratio of chlorophyll a to b than normal plants. We were able to substantiate these findings when plants were grown at 23 degrees C and lower (permissive temperatures). However, when grown at 26 degrees C (nonpermissive temperature) the plants produced sma...

The biosynthesis of coumarin in Melilotus alba.

Previous investigations have shown that CY4-labeled transcinnamic acid is rapidly converted to o-coumaric acid P-glucoside by shoots of Melilotus alba (white sweet clover) (1) and Hierochlod odorata (sweet grass) (2). This glucoside is then converted to coumarin (l-3), possibly by way of trans-cis isomerization of the glucoside to coumarinic acid P-glucoside. The small amount of radioactivity f...

Genetic Studies of Induced Mutants in Melilotus alba. II. Inheritance and Complementation of Chlorophyll-deficient Mutants

Six ethyl methanesulfonate-induced mutants of Melilotus alba Desr. were studied. Five of the mutants behaved as monogenic recessives. In the sixth mutant, two independent recessive alleles were responsible for the observed phenotype. Of these two genes, one had a phenotypic effect similar to the five single-gene mutants. The other (the veined gene) caused a chlorophyll deficiency in which the l...